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2016 n a hela s3 atcc  (ATCC)


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    Structured Review

    ATCC 2016 n a hela s3 atcc
    2016 N A Hela S3 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2016+n+a+hela+s3+atcc/HeLa+S3/pmc08221569__mmc4-238-73-77
    Average 97 stars, based on 1700 article reviews
    2016 n a hela s3 atcc - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies TONSL Sigma Cat no. HPA046494; RRID: AB_2679673 CAF1A/p150 Quivy et al., 2004 N/A MCM2 Bethyl Laboratories Cat no. A300-122A; RRID: AB_155897 CAF1B/p60 Quivy et al., 2004 N/A DNAJC9 AbCam Cat no. ab150394; RRID: AB_2890229 HA Cell Signaling Technology Cat no. C29F4 #3724; RRID: AB_1549585 DAXX Sigma Cat no. HPA008736; RRID: AB_1078625 HJURP Sigma Cat no. HPA008436; RRID: AB_1850757 Flag Sigma Cat no. F7425; RRID: AB_439687 H3 Abcam Cat no. ab1791; RRID: AB_302613 H4 Millipore Cat no. 05-858; RRID: AB_390138 Tubulin Abcam Cat no. ab6160; RRID: AB_305328 BAG2 Abcam Cat no. ab79406; RRID: AB_1603761 HSC/HSP70 Santa Cruz Biotechnology Cat no. sc-24; RRID: AB_627760 HSC70 Santa Cruz Biotechnology Cat no. sc-7298; RRID: AB_627761 Flag Sigma Cat no. F3165; RRID: AB_259529 Goat Anti-Mouse-Alexa488 Thermo Scientific Cat no. A-11029; RRID: AB_138404 Bacterial and virus strains E.coli: BL21 (DE3)-RIL Stratagene Cat no. 230245 Chemicals, peptides, and recombinant proteins Doxycycline Clontech Cat no. 631311 ULP1 protease Homemade N/A TEV protease Homemade N/A 3C protease Homemade N/A Drosophila topoisomerase I (dTopo I) Homemade N/A Micrococcal Nuclease Solution Thermo Scientific Cat no. 88216 Benzonase Nuclease Millipore Cat no. 70746 RNase A Sigma Cat no. R4875 Sequencing Grade Modified Trypsin Promega Cat no. V5113 Trypsin, Proteomics Grade Sigma Cat no. T6567 Critical commercial assays MinElute PCR Purification Kit QIAGEN Cat no. 28004 MinElute Reaction Cleanup Kit QIAGEN Cat no. 28204 truChIP Chromatin Shearing Kit Covaris Cat no. 520127 KAPA Hyperprep Kit Kappa Biosystems, Roche Cat no. KK8504 Deposited data Structure of the DNAJC9 aB–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CIZ Structure of the DNAJC9 HBD–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CJ0 ChIP-seq Data This study GEO: GSE154445 Raw Mass Spectrometry Data Sets This study PRIDE: PXD020268 (Continued on next page) Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw Data for Western Blots, Gel-based Assays and ITC This study Mendeley Data: https://doi.org/ 10.17632/y9mgyw9r59.1 Experimental models: Cell lines U-2-OS FRT/TO Flp-In T-REX Malecki et al., 2006 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-WT Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-Y81A-Y90A Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-WT Saredi et al., 2016 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-N571A Saredi et al., 2016 N/A HeLa S3 ATCC Cat no. CCL-2.2; RRID: CVCL_0058 HeLa S3 pTetOne Puro H3.1-FlagHA This study N/A HeLa S3 pTetOne Puro H3.2-FlagHA This study N/A HeLa S3 pTetOne Puro H3.3-FlagHA This study N/A HeLa S3 pTetOne Puro H4-FlagHA This study N/A HeLa S3 pTetOne Puro CENPA-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1T-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1-E105AD106A-FlagHA This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9MYC-Flag (WT) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-MYC-Flag (J) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9Q224A-R227A-M238A-Y242A-MYCFlag (4A) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-Q224A-R227A-M238AY242A-MYC-Flag (4AJ) This study N/A 293FT Thermo Scientific Cat no. R70007; RRID: CVCL_6911 Drosophila S2-DRSC cells Drosophila Genomics Resource Center; Stock No. 181 RRID: CVCL_Z992 Oligonucleotides NGS indexed PentAdapters PentaBase Cat no. SKU 310 Silencer Select siDNAJC9 Thermo Scientific Cat no. s23354 Silencer Select siBAG2 Thermo Scientific Cat no. s18295 Silencer Select siHSPA8 (siHSP7C) Thermo Scientific Cat no. s6987 Silencer Select Negative Control No. 1 Thermo Scientific Cat no. 4390843 Recombinant DNA FX174 RF I DNA NEB Cat no. N3021S pTetOne Puro H3.1-FlagHA This study N/A pTetOne Puro H3.2-FlagHA This study N/A pTetOne Puro H3.3-FlagHA This study N/A pTetOne Puro H4-FlagHA This study N/A pTetOne Puro CENPA-FlagHA This study N/A pTetOne Puro H3.1T-FlagHA This study N/A pTetOne Puro H3.1-E105A-D106A-FlagHA This study N/A (Continued on next page) ll OPEN ACCESS Article e2 Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV6-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pCMV6-DNAJC9-siRNA-resistent-L216AI220A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-Q224AR227A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-M238AY242A-MycFlag This study N/A pCMV6-DNAJC9-siRNA-resistent-E195AE199A- MycFlag This study N/A pcDNA5-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pcDNA5-DNAJC9-siRNA-resistentQ224A-R227A-M238A-Y242A-MycFlag (4A) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Myc-Flag (J) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Q224A-R227A-M238A-Y242A-MycFlag (4AJ) This study N/A Software and algorithms HKL-3000 Minor et al., 2006 https://www.hkl-xray.com/hkl-3000 Xia2 Winter, 2010 https://xia2.github.io/ Phenix Adams et al., 2010 https://www.phenix-online.org/ PyMOL The PyMOL Molecular Graphics System, Version 1.7, Schrodinger, LLC https://www.pymol.org/2/ MaxQuant 1.6.3.4 Cox and Mann, 2008; Cox et al., 2011 https://maxquant.net/maxquant/ Perseus 1.6.2.3 Tyanova et al., 2016 https://maxquant.net/perseus/ GraphPad Prism v8.4 GraphPad Software https://www.graphpad.com/scientific- software/prism/ R 3.6.2 The R Foundation https://cran.r-project.org/ Cytoscape 3.8.0 Shannon et al., 2003 https://cytoscape.org/ stringApp 1.5.1 Doncheva et al., 2019 http://apps.cytoscape.org/ Omics Visualizer app 1.3.0 Legeay et al., 2020 http://apps.cytoscape.org/ Trim Galore!

    Next-Generation Sequencing:

    Article Title: DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies TONSL Sigma Cat no. HPA046494; RRID: AB_2679673 CAF1A/p150 Quivy et al., 2004 N/A MCM2 Bethyl Laboratories Cat no. A300-122A; RRID: AB_155897 CAF1B/p60 Quivy et al., 2004 N/A DNAJC9 AbCam Cat no. ab150394; RRID: AB_2890229 HA Cell Signaling Technology Cat no. C29F4 #3724; RRID: AB_1549585 DAXX Sigma Cat no. HPA008736; RRID: AB_1078625 HJURP Sigma Cat no. HPA008436; RRID: AB_1850757 Flag Sigma Cat no. F7425; RRID: AB_439687 H3 Abcam Cat no. ab1791; RRID: AB_302613 H4 Millipore Cat no. 05-858; RRID: AB_390138 Tubulin Abcam Cat no. ab6160; RRID: AB_305328 BAG2 Abcam Cat no. ab79406; RRID: AB_1603761 HSC/HSP70 Santa Cruz Biotechnology Cat no. sc-24; RRID: AB_627760 HSC70 Santa Cruz Biotechnology Cat no. sc-7298; RRID: AB_627761 Flag Sigma Cat no. F3165; RRID: AB_259529 Goat Anti-Mouse-Alexa488 Thermo Scientific Cat no. A-11029; RRID: AB_138404 Bacterial and virus strains E.coli: BL21 (DE3)-RIL Stratagene Cat no. 230245 Chemicals, peptides, and recombinant proteins Doxycycline Clontech Cat no. 631311 ULP1 protease Homemade N/A TEV protease Homemade N/A 3C protease Homemade N/A Drosophila topoisomerase I (dTopo I) Homemade N/A Micrococcal Nuclease Solution Thermo Scientific Cat no. 88216 Benzonase Nuclease Millipore Cat no. 70746 RNase A Sigma Cat no. R4875 Sequencing Grade Modified Trypsin Promega Cat no. V5113 Trypsin, Proteomics Grade Sigma Cat no. T6567 Critical commercial assays MinElute PCR Purification Kit QIAGEN Cat no. 28004 MinElute Reaction Cleanup Kit QIAGEN Cat no. 28204 truChIP Chromatin Shearing Kit Covaris Cat no. 520127 KAPA Hyperprep Kit Kappa Biosystems, Roche Cat no. KK8504 Deposited data Structure of the DNAJC9 aB–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CIZ Structure of the DNAJC9 HBD–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CJ0 ChIP-seq Data This study GEO: GSE154445 Raw Mass Spectrometry Data Sets This study PRIDE: PXD020268 (Continued on next page) Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw Data for Western Blots, Gel-based Assays and ITC This study Mendeley Data: https://doi.org/ 10.17632/y9mgyw9r59.1 Experimental models: Cell lines U-2-OS FRT/TO Flp-In T-REX Malecki et al., 2006 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-WT Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-Y81A-Y90A Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-WT Saredi et al., 2016 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-N571A Saredi et al., 2016 N/A HeLa S3 ATCC Cat no. CCL-2.2; RRID: CVCL_0058 HeLa S3 pTetOne Puro H3.1-FlagHA This study N/A HeLa S3 pTetOne Puro H3.2-FlagHA This study N/A HeLa S3 pTetOne Puro H3.3-FlagHA This study N/A HeLa S3 pTetOne Puro H4-FlagHA This study N/A HeLa S3 pTetOne Puro CENPA-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1T-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1-E105AD106A-FlagHA This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9MYC-Flag (WT) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-MYC-Flag (J) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9Q224A-R227A-M238A-Y242A-MYCFlag (4A) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-Q224A-R227A-M238AY242A-MYC-Flag (4AJ) This study N/A 293FT Thermo Scientific Cat no. R70007; RRID: CVCL_6911 Drosophila S2-DRSC cells Drosophila Genomics Resource Center; Stock No. 181 RRID: CVCL_Z992 Oligonucleotides NGS indexed PentAdapters PentaBase Cat no. SKU 310 Silencer Select siDNAJC9 Thermo Scientific Cat no. s23354 Silencer Select siBAG2 Thermo Scientific Cat no. s18295 Silencer Select siHSPA8 (siHSP7C) Thermo Scientific Cat no. s6987 Silencer Select Negative Control No. 1 Thermo Scientific Cat no. 4390843 Recombinant DNA FX174 RF I DNA NEB Cat no. N3021S pTetOne Puro H3.1-FlagHA This study N/A pTetOne Puro H3.2-FlagHA This study N/A pTetOne Puro H3.3-FlagHA This study N/A pTetOne Puro H4-FlagHA This study N/A pTetOne Puro CENPA-FlagHA This study N/A pTetOne Puro H3.1T-FlagHA This study N/A pTetOne Puro H3.1-E105A-D106A-FlagHA This study N/A (Continued on next page) ll OPEN ACCESS Article e2 Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV6-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pCMV6-DNAJC9-siRNA-resistent-L216AI220A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-Q224AR227A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-M238AY242A-MycFlag This study N/A pCMV6-DNAJC9-siRNA-resistent-E195AE199A- MycFlag This study N/A pcDNA5-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pcDNA5-DNAJC9-siRNA-resistentQ224A-R227A-M238A-Y242A-MycFlag (4A) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Myc-Flag (J) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Q224A-R227A-M238A-Y242A-MycFlag (4AJ) This study N/A Software and algorithms HKL-3000 Minor et al., 2006 https://www.hkl-xray.com/hkl-3000 Xia2 Winter, 2010 https://xia2.github.io/ Phenix Adams et al., 2010 https://www.phenix-online.org/ PyMOL The PyMOL Molecular Graphics System, Version 1.7, Schrodinger, LLC https://www.pymol.org/2/ MaxQuant 1.6.3.4 Cox and Mann, 2008; Cox et al., 2011 https://maxquant.net/maxquant/ Perseus 1.6.2.3 Tyanova et al., 2016 https://maxquant.net/perseus/ GraphPad Prism v8.4 GraphPad Software https://www.graphpad.com/scientific- software/prism/ R 3.6.2 The R Foundation https://cran.r-project.org/ Cytoscape 3.8.0 Shannon et al., 2003 https://cytoscape.org/ stringApp 1.5.1 Doncheva et al., 2019 http://apps.cytoscape.org/ Omics Visualizer app 1.3.0 Legeay et al., 2020 http://apps.cytoscape.org/ Trim Galore!

    Negative Control:

    Article Title: DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies TONSL Sigma Cat no. HPA046494; RRID: AB_2679673 CAF1A/p150 Quivy et al., 2004 N/A MCM2 Bethyl Laboratories Cat no. A300-122A; RRID: AB_155897 CAF1B/p60 Quivy et al., 2004 N/A DNAJC9 AbCam Cat no. ab150394; RRID: AB_2890229 HA Cell Signaling Technology Cat no. C29F4 #3724; RRID: AB_1549585 DAXX Sigma Cat no. HPA008736; RRID: AB_1078625 HJURP Sigma Cat no. HPA008436; RRID: AB_1850757 Flag Sigma Cat no. F7425; RRID: AB_439687 H3 Abcam Cat no. ab1791; RRID: AB_302613 H4 Millipore Cat no. 05-858; RRID: AB_390138 Tubulin Abcam Cat no. ab6160; RRID: AB_305328 BAG2 Abcam Cat no. ab79406; RRID: AB_1603761 HSC/HSP70 Santa Cruz Biotechnology Cat no. sc-24; RRID: AB_627760 HSC70 Santa Cruz Biotechnology Cat no. sc-7298; RRID: AB_627761 Flag Sigma Cat no. F3165; RRID: AB_259529 Goat Anti-Mouse-Alexa488 Thermo Scientific Cat no. A-11029; RRID: AB_138404 Bacterial and virus strains E.coli: BL21 (DE3)-RIL Stratagene Cat no. 230245 Chemicals, peptides, and recombinant proteins Doxycycline Clontech Cat no. 631311 ULP1 protease Homemade N/A TEV protease Homemade N/A 3C protease Homemade N/A Drosophila topoisomerase I (dTopo I) Homemade N/A Micrococcal Nuclease Solution Thermo Scientific Cat no. 88216 Benzonase Nuclease Millipore Cat no. 70746 RNase A Sigma Cat no. R4875 Sequencing Grade Modified Trypsin Promega Cat no. V5113 Trypsin, Proteomics Grade Sigma Cat no. T6567 Critical commercial assays MinElute PCR Purification Kit QIAGEN Cat no. 28004 MinElute Reaction Cleanup Kit QIAGEN Cat no. 28204 truChIP Chromatin Shearing Kit Covaris Cat no. 520127 KAPA Hyperprep Kit Kappa Biosystems, Roche Cat no. KK8504 Deposited data Structure of the DNAJC9 aB–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CIZ Structure of the DNAJC9 HBD–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CJ0 ChIP-seq Data This study GEO: GSE154445 Raw Mass Spectrometry Data Sets This study PRIDE: PXD020268 (Continued on next page) Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw Data for Western Blots, Gel-based Assays and ITC This study Mendeley Data: https://doi.org/ 10.17632/y9mgyw9r59.1 Experimental models: Cell lines U-2-OS FRT/TO Flp-In T-REX Malecki et al., 2006 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-WT Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-Y81A-Y90A Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-WT Saredi et al., 2016 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-N571A Saredi et al., 2016 N/A HeLa S3 ATCC Cat no. CCL-2.2; RRID: CVCL_0058 HeLa S3 pTetOne Puro H3.1-FlagHA This study N/A HeLa S3 pTetOne Puro H3.2-FlagHA This study N/A HeLa S3 pTetOne Puro H3.3-FlagHA This study N/A HeLa S3 pTetOne Puro H4-FlagHA This study N/A HeLa S3 pTetOne Puro CENPA-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1T-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1-E105AD106A-FlagHA This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9MYC-Flag (WT) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-MYC-Flag (J) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9Q224A-R227A-M238A-Y242A-MYCFlag (4A) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-Q224A-R227A-M238AY242A-MYC-Flag (4AJ) This study N/A 293FT Thermo Scientific Cat no. R70007; RRID: CVCL_6911 Drosophila S2-DRSC cells Drosophila Genomics Resource Center; Stock No. 181 RRID: CVCL_Z992 Oligonucleotides NGS indexed PentAdapters PentaBase Cat no. SKU 310 Silencer Select siDNAJC9 Thermo Scientific Cat no. s23354 Silencer Select siBAG2 Thermo Scientific Cat no. s18295 Silencer Select siHSPA8 (siHSP7C) Thermo Scientific Cat no. s6987 Silencer Select Negative Control No. 1 Thermo Scientific Cat no. 4390843 Recombinant DNA FX174 RF I DNA NEB Cat no. N3021S pTetOne Puro H3.1-FlagHA This study N/A pTetOne Puro H3.2-FlagHA This study N/A pTetOne Puro H3.3-FlagHA This study N/A pTetOne Puro H4-FlagHA This study N/A pTetOne Puro CENPA-FlagHA This study N/A pTetOne Puro H3.1T-FlagHA This study N/A pTetOne Puro H3.1-E105A-D106A-FlagHA This study N/A (Continued on next page) ll OPEN ACCESS Article e2 Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV6-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pCMV6-DNAJC9-siRNA-resistent-L216AI220A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-Q224AR227A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-M238AY242A-MycFlag This study N/A pCMV6-DNAJC9-siRNA-resistent-E195AE199A- MycFlag This study N/A pcDNA5-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pcDNA5-DNAJC9-siRNA-resistentQ224A-R227A-M238A-Y242A-MycFlag (4A) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Myc-Flag (J) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Q224A-R227A-M238A-Y242A-MycFlag (4AJ) This study N/A Software and algorithms HKL-3000 Minor et al., 2006 https://www.hkl-xray.com/hkl-3000 Xia2 Winter, 2010 https://xia2.github.io/ Phenix Adams et al., 2010 https://www.phenix-online.org/ PyMOL The PyMOL Molecular Graphics System, Version 1.7, Schrodinger, LLC https://www.pymol.org/2/ MaxQuant 1.6.3.4 Cox and Mann, 2008; Cox et al., 2011 https://maxquant.net/maxquant/ Perseus 1.6.2.3 Tyanova et al., 2016 https://maxquant.net/perseus/ GraphPad Prism v8.4 GraphPad Software https://www.graphpad.com/scientific- software/prism/ R 3.6.2 The R Foundation https://cran.r-project.org/ Cytoscape 3.8.0 Shannon et al., 2003 https://cytoscape.org/ stringApp 1.5.1 Doncheva et al., 2019 http://apps.cytoscape.org/ Omics Visualizer app 1.3.0 Legeay et al., 2020 http://apps.cytoscape.org/ Trim Galore!

    Recombinant:

    Article Title: DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies TONSL Sigma Cat no. HPA046494; RRID: AB_2679673 CAF1A/p150 Quivy et al., 2004 N/A MCM2 Bethyl Laboratories Cat no. A300-122A; RRID: AB_155897 CAF1B/p60 Quivy et al., 2004 N/A DNAJC9 AbCam Cat no. ab150394; RRID: AB_2890229 HA Cell Signaling Technology Cat no. C29F4 #3724; RRID: AB_1549585 DAXX Sigma Cat no. HPA008736; RRID: AB_1078625 HJURP Sigma Cat no. HPA008436; RRID: AB_1850757 Flag Sigma Cat no. F7425; RRID: AB_439687 H3 Abcam Cat no. ab1791; RRID: AB_302613 H4 Millipore Cat no. 05-858; RRID: AB_390138 Tubulin Abcam Cat no. ab6160; RRID: AB_305328 BAG2 Abcam Cat no. ab79406; RRID: AB_1603761 HSC/HSP70 Santa Cruz Biotechnology Cat no. sc-24; RRID: AB_627760 HSC70 Santa Cruz Biotechnology Cat no. sc-7298; RRID: AB_627761 Flag Sigma Cat no. F3165; RRID: AB_259529 Goat Anti-Mouse-Alexa488 Thermo Scientific Cat no. A-11029; RRID: AB_138404 Bacterial and virus strains E.coli: BL21 (DE3)-RIL Stratagene Cat no. 230245 Chemicals, peptides, and recombinant proteins Doxycycline Clontech Cat no. 631311 ULP1 protease Homemade N/A TEV protease Homemade N/A 3C protease Homemade N/A Drosophila topoisomerase I (dTopo I) Homemade N/A Micrococcal Nuclease Solution Thermo Scientific Cat no. 88216 Benzonase Nuclease Millipore Cat no. 70746 RNase A Sigma Cat no. R4875 Sequencing Grade Modified Trypsin Promega Cat no. V5113 Trypsin, Proteomics Grade Sigma Cat no. T6567 Critical commercial assays MinElute PCR Purification Kit QIAGEN Cat no. 28004 MinElute Reaction Cleanup Kit QIAGEN Cat no. 28204 truChIP Chromatin Shearing Kit Covaris Cat no. 520127 KAPA Hyperprep Kit Kappa Biosystems, Roche Cat no. KK8504 Deposited data Structure of the DNAJC9 aB–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CIZ Structure of the DNAJC9 HBD–H3.3–H4– MCM2 HBD quaternary complex This study PDB: 7CJ0 ChIP-seq Data This study GEO: GSE154445 Raw Mass Spectrometry Data Sets This study PRIDE: PXD020268 (Continued on next page) Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Raw Data for Western Blots, Gel-based Assays and ITC This study Mendeley Data: https://doi.org/ 10.17632/y9mgyw9r59.1 Experimental models: Cell lines U-2-OS FRT/TO Flp-In T-REX Malecki et al., 2006 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-WT Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX FlagHAMCM2-Y81A-Y90A Huang et al., 2015 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-WT Saredi et al., 2016 N/A U-2-OS FRT/TO Flp-In T-REX U-2-OS eGFP-TONSL-N571A Saredi et al., 2016 N/A HeLa S3 ATCC Cat no. CCL-2.2; RRID: CVCL_0058 HeLa S3 pTetOne Puro H3.1-FlagHA This study N/A HeLa S3 pTetOne Puro H3.2-FlagHA This study N/A HeLa S3 pTetOne Puro H3.3-FlagHA This study N/A HeLa S3 pTetOne Puro H4-FlagHA This study N/A HeLa S3 pTetOne Puro CENPA-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1T-FlagHA This study N/A HeLa S3 pTetOne Puro H3.1-E105AD106A-FlagHA This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9MYC-Flag (WT) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-MYC-Flag (J) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9Q224A-R227A-M238A-Y242A-MYCFlag (4A) This study N/A U-2-OS FRT/TO Flp-In T-REX DNAJC9H43Q-D45N-Q224A-R227A-M238AY242A-MYC-Flag (4AJ) This study N/A 293FT Thermo Scientific Cat no. R70007; RRID: CVCL_6911 Drosophila S2-DRSC cells Drosophila Genomics Resource Center; Stock No. 181 RRID: CVCL_Z992 Oligonucleotides NGS indexed PentAdapters PentaBase Cat no. SKU 310 Silencer Select siDNAJC9 Thermo Scientific Cat no. s23354 Silencer Select siBAG2 Thermo Scientific Cat no. s18295 Silencer Select siHSPA8 (siHSP7C) Thermo Scientific Cat no. s6987 Silencer Select Negative Control No. 1 Thermo Scientific Cat no. 4390843 Recombinant DNA FX174 RF I DNA NEB Cat no. N3021S pTetOne Puro H3.1-FlagHA This study N/A pTetOne Puro H3.2-FlagHA This study N/A pTetOne Puro H3.3-FlagHA This study N/A pTetOne Puro H4-FlagHA This study N/A pTetOne Puro CENPA-FlagHA This study N/A pTetOne Puro H3.1T-FlagHA This study N/A pTetOne Puro H3.1-E105A-D106A-FlagHA This study N/A (Continued on next page) ll OPEN ACCESS Article e2 Molecular Cell 81, 2533–2548.e1–e9, June 17, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV6-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pCMV6-DNAJC9-siRNA-resistent-L216AI220A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-Q224AR227A-Myc-Flag This study N/A pCMV6-DNAJC9-siRNA-resistent-M238AY242A-MycFlag This study N/A pCMV6-DNAJC9-siRNA-resistent-E195AE199A- MycFlag This study N/A pcDNA5-DNAJC9-siRNA-resistent-MycFlag (WT) This study N/A pcDNA5-DNAJC9-siRNA-resistentQ224A-R227A-M238A-Y242A-MycFlag (4A) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Myc-Flag (J) This study N/A pcDNA5-DNAJC9-siRNA-resistent-H43QD45N-Q224A-R227A-M238A-Y242A-MycFlag (4AJ) This study N/A Software and algorithms HKL-3000 Minor et al., 2006 https://www.hkl-xray.com/hkl-3000 Xia2 Winter, 2010 https://xia2.github.io/ Phenix Adams et al., 2010 https://www.phenix-online.org/ PyMOL The PyMOL Molecular Graphics System, Version 1.7, Schrodinger, LLC https://www.pymol.org/2/ MaxQuant 1.6.3.4 Cox and Mann, 2008; Cox et al., 2011 https://maxquant.net/maxquant/ Perseus 1.6.2.3 Tyanova et al., 2016 https://maxquant.net/perseus/ GraphPad Prism v8.4 GraphPad Software https://www.graphpad.com/scientific- software/prism/ R 3.6.2 The R Foundation https://cran.r-project.org/ Cytoscape 3.8.0 Shannon et al., 2003 https://cytoscape.org/ stringApp 1.5.1 Doncheva et al., 2019 http://apps.cytoscape.org/ Omics Visualizer app 1.3.0 Legeay et al., 2020 http://apps.cytoscape.org/ Trim Galore!



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